Inside cells, communication between the nucleus, which harbours our precious genetic material, and the cytoplasm is mediated by the constant exchange of thousands of signaling molecules and proteins. Until now, it was unknown how this protein traffic can be so fast and yet precise enough to prevent the passage of unwanted molecules. Through a combination of computer simulations and various experimental techniques, researchers from Germany, France and the UK have solved this puzzle: A very flexible and disordered protein can bind to its receptor within billionths of a second. Their research, led by Edward Lemke (EMBL), Frauke Gräter (HITS), and Martin Blackledge (IBS) is published in “Cell” this week.
Proteins can recognize one another. Each engages very specifically with only a subset of the many different proteins present in the living cell, like a key slotting into a lock. But what if the key is completely flexible, as is the case for so-called intrinsically disordered proteins (IDPs)? The research teams headed by Edward Lemke at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Frauke Gräter at the Heidelberg Institute for Theoretical Studies (HITS) and Martin Blackledge at the Institut de Biologie Structurale, (IBS) in France, addressed this question in a highly interdisciplinary collaboration, combining molecular simulations, single molecule fluorescence resonance energy transfer (FRET), nuclear magnetic resonance (NMR), stopped flow spectroscopy and in-cell particle tracking.
Unexpectedly, they found that flexible, spaghetti-like proteins can be good – maybe even better than solid protein blocks – at being recognised by multiple partners. And they can do so very fast, while still retaining the high specificity the cell needs. In fact, this could be why these disordered molecules are more common in evolutionarily higher organisms, the researchers surmise.
Researchers had assumed that when an IDP ‘key’ needed to bind to its lock, it rearranged itself to become more rigid, but experiments in the Lemke lab hinted otherwise. “The pioneering single molecule experiments undertaken at EMBL showed for the particular interaction of a receptor with a disordered protein just nothing: the flexible protein stayed as flexible even when bound to its receptor” says Davide Mercadante (HITS). This prompted him to study the very same interaction on the computer. The surprising result was that the high flexibility of the IDP actually helps it bind to its lock – in this case, a nuclear transport receptor, which shuttles proteins into the nucleus. The simulations even suggested the binding to be ultrafast – faster than any other association of that kind recorded to date. “The computational data indicated that we might have identified a new ultrafast binding mechanism, but it took us three years to design experiments to prove the kinetics in the lab,” Iker Valle Aramburu (EMBL) recalls. “In the end, we had a remarkably perfect match.”
The results now help to understand a long-standing paradox: “For a cell to be viable, molecules must constantly move into and out of its nucleus”, says Edward Lemke (EMBL). ”Our findings explain the so-called transport paradox – that is, how this shuttling can be so very fast while remaining specific so that unwanted molecules cannot pass the barrier that protects our genome.”
The new study suggests that many binding motifs at the surface of the IDP create a highly reactive surface that together with the very high speed of locking and unlocking ensures efficient proof-reading while the receptors to travel so fast through a pore filled with other IDPs.
“This is likely a new paradigm for the recognition of intrinsically disordered proteins.” says Frauke Gräter (HITS). Since around 30-50% of the proteins in human cells are disordered, at least in some regions of the protein, the results may also provide a rationale for how recognition information can be processed very fast in general – which is vital to cells.
Other researchers involved in the study are working at the IBS Grenoble / France, and Cambridge University / UK.
See also EMBL press release: http://s.embl.org/pr081015
Publication in “Cell”: Plasticity of an ultrafast interaction between nucleoporins and nuclear transport receptors
Sigrid Milles, Davide Mercadante, Iker Valle Aramburu, Malene Ringkjøbing Jensen, Niccolò Banterle, Christine Koehler, Swati Tyagi, Jane Clarke, Sarah L Shammas, Martin Blackledge, Frauke Gräter, Edward A Lemke
Sonia Furtado Neves
EMBL Press Officer & Deputy Head of Communications
Tel.: +49 6221 387 8263
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Dr. Peter Saueressig
Head of Communications
Heidelberg Institute for Theoretical Studies (HITS)
Prof. Dr. Frauke Gräter
Heidelberg Institute for Theoretical Studies (HITS)
Dr. Edward Lemke
Structural and Computational Biology Unit, Cell Biology and Biophysics Unit, (EMBL)
Phone: +49-6221-387 8536
HITS, the Heidelberg Institute for Theoretical Studies, was established in 2010 by physicist and SAP co-founder Klaus Tschira (1940-2015) and the Klaus Tschira Foundation as a private, non-profit research institute. HITS conducts basic research in the natural, mathematical, and computer sciences. Major research directions include complex simulations across scales, making sense of data, and enabling science via computational research. Application areas range from molecular biology to astrophysics. An essential characteristic of the Institute is interdisciplinarity, implemented in numerous cross-group and cross-disciplinary projects. The base funding of HITS is provided by the Klaus Tschira Foundation.