Spindle pole body bridge on the front cover

13. August 2015
JCB_figure8c

HITS scientists Musa Özboyaci and Prof. Rebecca Wade (MCM group) have performed calculations that help to understand the process of cell division in yeast. In a multidisciplinary study conducted together with researchers at the Center for Molecular Biology of Heidelberg University (ZMBH) and at Frankfurt University, the team led by Prof. Elmar Schiebel (ZMBH) revealed the function of the Kar1 and Cdc31 proteins, crucial components of the yeast spindle pole body (SPB), in SPB duplication. The duplication of the SPB is an essential part of the chromosome segregation process in every cell cycle. At the beginning of the cell division process and SPB duplication, a multiprotein structure called the half-bridge juts out from the SPB and elongates into a bridge structure. In this study, the HITS researchers conducted free energy calculations which indicated that the Cdc31 proteins stabilize the structure of the SPB bridge to promote its duplication. This mechanism was supported by yeast growth measurements for a set of Cdc31 mutants. The SPB is the functional equivalent of the mammalian centrosome and thus these results improve our basic understanding of cell division processes which are of fundamental importance in cancer research. The article was published in the Journal of Cell Biology (Seybold et al. (2015), J. Cell. Biol. 209, 843-861, doi:10.1083/jcb.201412050) together with an electron microscopy image illustrating the role of the Kar1 protein in supporting the SPB bridge on the front cover.

About HITS

The Heidelberg Institute for Theoretical Studies (HITS) was established in 2010 by the physicist and SAP co-founder Klaus Tschira (1940-2015) and the Klaus Tschira Foundation as a private, non-profit research institute. HITS conducts basic research in the natural sciences, mathematics and computer science, with a focus on the processing, structuring, and analyzing of large amounts of complex data and the development of computational methods and software. The research fields range from molecular biology to astrophysics. The shareholders of HITS are the HITS-Stiftung, which is a subsidiary of the Klaus Tschira Foundation, Heidelberg University and the Karlsruhe Institute of Technology (KIT). HITS also cooperates with other universities and research institutes and with industrial partners. The base funding of HITS is provided by the HITS Stiftung with funds received from the Klaus Tschira Foundation. The primary external funding agencies are the Federal Ministry of Education and Research (BMBF), the German Research Foundation (DFG), and the European Union.