Stretched beyond the limits

8. May 2020

It is a common phenomenon we know from cracked sneakers and burst tyres, and when it occurs it is usually in the wrong place at the wrong time: worn-out materials can cause anything from mild annoyance to fatal accidents. But while fatigue is well understood in synthetic materials, we know much less about such processes in mammalian tissue, which often needs to cope with extreme mechanical stresses as well. An international team led by researchers at the Heidelberg Institute for Theoretical Studies (HITS) has now shown that mechanical stress can similarly deteriorate collagen tissue. The findings, published in Nature Communications, might help to advance material research and biomedicine.

It has been known for many decades that synthetic polymers subjected to mechanical stress generate mechanoradicals by rupture of chemical bonds. But could those harmful and highly reactive radicals also form in our tissues when stretched? Scientists from the Molecular Biomechanics Group at HITS tackled this question by taking a closer look at collagen, the protein which holds us together – literally – and provides structural and mechanical stability to all our connective tissues like bones, tendons, ligaments, and skin. “In this role it is under perpetual mechanical load and as such the perfect candidate”, says Frauke Gräter, who led the research at HITS. Together with colleagues from Homburg, Frankfurt and Seattle, her team showed in a series of especially devised experiments that excessive mechanical stress on collagen produces radicals. Knowing that radicals are known to cause damage and oxidative stress in the body, this finding was critical forthe researchers.

“We managed to mount and pull a rat tail fascicle directly in the Electron-paramagnetic resonance cavity to monitor radical formation due to force in real time”, explains Christopher Zapp, PhD student in Graeter’s team, the experimental set-up. Additional Molecular Dynamics simulations of the collagen fibril, comprising millions of atoms, helped to explain the observations: Chemical bonds break when collagen is stretched. But the resulting harmful radicals are quickly scavenged by nearby aromatic residues, so-called DOPAs. “Not only did we find stable radicals in collagen tissue, we also discovered DOPA residues in collagen, a modification that protects collagen against further damage.” The DOPA radicals then finally convert into hydrogen peroxide, an important oxidative molecule in the body. Collagen is therefore not just a mere bearer of force, it can also control its consequences.

“It was a challenging task to make sense of the peculiar radical signal we observed in the stressed biomaterial”, adds Reinhard Kappl from the Department of Biophysics at Saarland University and co-author of the study. “It needed the combination of expertise from different labs for a consistent picture.”

The study suggests that collagen has evolved as a radical sponge to combat damage. “We show that collagen protects itself from the radicals. Still, stretching this mechanism beyond its limits can eventually lead to oxidation-mediated pathologies, from pain to inflammation”, explains Agnieszka Obarska-Kosinska from HITS.

The findings might not only explain why playing football can at times be really painful, they are also a promising starting point for improving tissue repair and transplantation, for example in sports medicine.

Atomistic model of collagen I fibril with crosslinks (blue) that connect helices. Newly discovered DOPAs are shown in red.
Atomistic model of collagen I fibril with crosslinks (blue) that connect helices. Newly discovered DOPAs are shown in green and other residues potentially involved in scavenging oxidative stress in yellow, orange and red.

Paper: Nature Communications https://www.nature.com/articles/s41467-020-15567-4 (DOI https://doi.org/10.1038/s41467-020-15567-4)

Press Contact:
Dr. Peter Saueressig
Public Relations
Heidelberg Institute for Theoretical Studies (HITS)
Phone: +49 6221 533 245
Peter.Saueressig@h-its.org
https://www.h-its.org

Scientific Contact:
Prof. Dr. Frauke Gräter
Group Leader Molecular Biomechanics (MBM)
Heidelberg Institute for Theoretical Studies (HITS)
Phone: +49 6221 533 267
Frauke.Graeter@h-its.org

About HITS

The Heidelberg Institute for Theoretical Studies (HITS) was established in 2010 by the physicist and SAP co-founder Klaus Tschira (1940-2015) and the Klaus Tschira Foundation as a private, non-profit research institute. HITS conducts basic research in the natural sciences, mathematics and computer science, with a focus on the processing, structuring, and analyzing of large amounts of complex data and the development of computational methods and software. The research fields range from molecular biology to astrophysics. The shareholders of HITS are the HITS-Stiftung, which is a subsidiary of the Klaus Tschira Foundation, Heidelberg University and the Karlsruhe Institute of Technology (KIT). HITS also cooperates with other universities and research institutes and with industrial partners. The base funding of HITS is provided by the HITS Stiftung with funds received from the Klaus Tschira Foundation. The primary external funding agencies are the Federal Ministry of Education and Research (BMBF), the German Research Foundation (DFG), and the European Union.

Switch to the German homepage or stay on this page