Build your collagen fibril!


Obtain your atomistic collagen fibril models with ColBuilder! We have now published a web resource of collagen models for 20 different species with a large variety of crosslink types and localization within the fibril, to facilitate structure-based analyses and simulations of type I collagen in health and disease. To easily enable simulations, we provide parameters of the modeled crosslinks for an AMBER force field. This resource developed at HITS has now been published in „Biophysical Journal“. The repository of collagen models is available at

Type I collagen is the main structural component of many tissues in the human body. It provides excellent mechanical properties to connective tissue and acts as a protein interaction hub. There is thus a wide interest in understanding the properties and diverse functions of type I collagen at the molecular level. A precondition is an atomistic collagen I structure as it occurs in native tissue. To this end, we built full-atom models of crosslinked collagen fibrils by integrating the low-resolution structure of collagen fibril available from X-ray fiber diffraction with high-resolution structures of short collagen-like peptides from X-ray crystallography and mass spectrometry data.

The obtained models are made available through an interactive web server, which allows to choose between 20 different species, 8 different crosslink types and crosslink composition (individual choices among crosslink types at different collagen sites within the same model). On the one hand, users can download PDB-files containing models of the complete 300 nm single triple helix, which include the symmetry record allowing for the generation of collagen fibril bundles of any size. On the other hand, we also provide models of predefined bundles of collagen fibril fragments, comprising 41 triple helices, spanning one central overlap and one gap region (the latter in two parts) as shown in the Figure below. These models of fibril bundles are ready to use in MD simulations e.g. using GROMACS. To this end, a tar archive with a complete modified Amber99SB*-ildnp force field can be downloaded. Additionally, this archive contains a directory with only the additional crosslink parameters, which in turn can then be integrated into any Amber99 force field as well.

The repository of collagen models is available at

Atomistic model of a Collagen type 1 fibril fragment as obtained from ColBuilder. It comprises 41 triple helices spanning one central overlap and one gap region (in two parts, left and right). Triple helices are colored according to the staggering distance, crosslinks are shown in blue.

Article in Biophysical Journal (download here):

ColBuilder: a server to build collagen fibril models
Agnieszka Obarska-Kosinska, Benedikt Rennekamp, Aysecan Ünal and Frauke Gräter
Biophysical Journal

Scientific Contact:

Prof. Dr. Frauke Gräter
Group Leader „Molecular Biomechanics“
HITS – Heidelberg Institute for Theoretical Studies

Über das HITS

Das Heidelberger Institut für Theoretische Studien (HITS) wurde 2010 von dem Physiker und SAP-Mitgründer Klaus Tschira (1940-2015) und der Klaus Tschira Stiftung als private, gemeinnützige Forschungseinrichtung ins Leben gerufen. Das HITS betreibt Grundlagenforschung in den Naturwissenschaften, der Mathematik und der Informatik. Dabei werden große, komplexe Datenmengen verarbeitet, strukturiert und analysiert und computergestützte Methoden und Software entwickelt. Die Forschungsfelder reichen von der Molekularbiologie bis zur Astrophysik. Die HITS Stiftung, eine Tochter der Klaus Tschira Stiftung, stellt die Grundfinanzierung der HITS gGmbH auf Dauer sicher. Die Mittel dafür erhält sie von der Klaus Tschira Stiftung. Gesellschafter des HITS sind neben der HITS Stiftung die Universität Heidelberg und das Karlsruher Institut für Technologie (KIT). Das HITS arbeitet außerdem mit weiteren Universitäten und Forschungsinstituten sowie mit industriellen Partnern zusammen. Die wichtigsten externen Mittelgeber sind das Bundesministerium für Bildung und Forschung (BMBF), die Deutsche Forschungsgemeinschaft (DFG) und die Europäische Union.

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